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Image Search Results
Journal: Cell reports
Article Title: Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells
doi: 10.1016/j.celrep.2018.10.074
Figure Lengend Snippet: (A) Naive CD4 + T cells were transfected with either scrambled control or LNA21. Representative western blots and mean normalized band intensities of PTEN, SPRY1, and b-actin expression after 48 hr are shown (n = 3–4, mean ± SEM). (B and C) Naive CD4 + T cells from young and older individuals were activated with anti-CD3 and anti-CD28 beads and transduced with a lentiviral vector expressing scrambled control RNA or anti-miR-21. (B) Representative histogram of phosphorylated S6 in GFP + cells on day 2 and results from 7 individuals. (C) Representative histograms of phosphorylated S6, AKT, mTOR, and ERK in GFP + cells on day 3 and results of paired samples from 7 individuals. The filled gray histograms represent unstimulated naive CD4 + T cells. (D and E) Naive CD4 + T cells from healthy adults were activated with anti-CD3 and anti-CD28 beads. On day 3, activated cells were treated with combinations of the AKT inhibitor MK-2206 2HCl and the MEK1 and MEK2 inhibitor U0126 for 1.5 hr. (D) Representative histograms show phosphorylated S6, mTOR, and ERK in cells treated with combinations of 40 nM of the AKT inhibitor and 400 nM of MEK1 and MEK2 inhibitor. Numbers in histograms indicate geometric MFI of p-mTOR and p-ERK. (E) Graphs depicting the frequencies of cells with phosphorylated S6 cultured with the indicated concentrations of the AKT inhibitor in the presence or absence of 400 nM of the MEK1 and MEK2 inhibitor (n = 4, mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; all by two-tailed paired t test.
Article Snippet:
Techniques: Transfection, Western Blot, Expressing, Transduction, Plasmid Preparation, Cell Culture, Two Tailed Test
Journal: Cell reports
Article Title: Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells
doi: 10.1016/j.celrep.2018.10.074
Figure Lengend Snippet:
Article Snippet:
Techniques: Plasmid Preparation, Recombinant, Isolation, SYBR Green Assay, Reporter Assay, Microarray, Luciferase, Software